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了解一下该如何培养质粒吧
浏览次数:107发布日期:2020-04-03
   在基因工程中,常用人工构建的质粒作为载体。人工构建的质粒可以集多种有用的特征于一体,如含多种单一酶切位点、抗生素耐药性等。常用的人工质粒运载体有pBR322、pSC101。

懂球帝  pBR322HANYOUKANGSIHUANSUJIYIN(Tcr)HEKANGANBIANQINGMEISUJIYIN(Apr),BINGHANYOU27ZHONGXIANZHIXINGNEIQIEMEIDEDANYISHIBIEWEIDIAN。RUGUOJIANGDNAPIANDUANCHARUEcoRIQIEDIAN,BUHUIYINGXIANGLIANGGEKANGSHENGSUJIYINDEBIAODA。

  DANSHIRUGUOJIANGDNAPIANDUANCHARUDAOHind Ⅲ、Bam H I HUO Sal IQIEDIAN,JIUHUISHIKANGSIHUANSUJIYINSHIHUO。ZHEISHI,HANYOUDNACHARUPIANDUANDEpBR322JIANGSHISUZHUXIJUNKANGANBIANQINGMEISU,DANDUISIHUANSUMINGAN。

  MEIYOUDNACHARUPIANDUANDEpBR322HUISHISUZHUXIJUNJIKANGANBIANQINGMEISUYOUKANGSIHUANSU,ERMEIYOUpBR322ZHILIDEXIJUNJIANGDUIANBIANQINGMEISUHESIHUANSUDOUMINGAN。pSC101YUpBR322XIANGSI,ZHISHIMEIYOUKANGANBIANQINGMEISUJIYINHEPstIQIEDIAN。

  ZHILIYUNZAITIDEDACHARUPIANDUANYUEWEI10 kb(kbBIAOSHIWEIQIANJIANJIDUI)。

  1973NIAN,KEXUEJIAJIANGZHILIZUOWEIJIYINDEZAITISHIYONG,WEIJIYINGONGCHENGDEDANSHENGDIANDINGLEJICHU。

  CHANGYONGDEZHILISHIDACHANGGANJUNDEZHILI。ZHEIZHONGZHILICHANGHANYOUKANGSHENGSUKANGXINGJIYIN,LIRU,KANEIMEISUKANGXINGJIYIN。

懂球帝  pBR322ZHILIDNAFENZIDEZHANGDUWEI4361bp*(*Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported.),CIZAITIZHONGYOULIANGGEBIAOJIJIYIN,YIGESHIANBIANQINGMEISUKANGXINGJIYIN(Apr),LINGYIGESHISIHUANSUKANGXINGJIYIN(Tetr)。YIZHIpBR322DNAFENZIGONGYOU24ZHONGHESUANNEIQIEXIANZHIMEIDEDANYISHIBIEWEIDIAN。

  QIZHONG7ZHONGXIANZHIMEI(CONG12:00WEIZHIANSHUNSHIZHENFANGXIANG)JIEcoRV、NheI、BamHI、SphI、SalI、XmaⅢHENruIDESHIBIEWEIDIANWEIYUSIHUANSUKANGXINGJIYINNEIBU,LINGWAIYOU2 ZHONGXIANZHIMEIJIClaIHEHindⅢDESHIBIEWEIDIANSHICUNZAIYUZHEIGEJIYINDEQIDONGQUNEI,ZAIZHEI9GEXIANZHIWEIDIANSHANGCHARUWAIYUANDNADOUHUIDAOZHItetrDESHIHUO。

  3ZHONGXIANZHIMEIJIScaI、PvuIHEPstIDESHIBIEWEIDIANWEIYUANBIANQINGMEISUKANGXINGJIYINNEI,ZAIZHEIXIEWEIDIANCHARUWAIYUANDNAZEHUIDAOZHIamprJIYINDESHIHUO。YOUZHILIZAITIDEJIEGOUKEZHIQIJUYOURUXIAYOUDIAN:

懂球帝  ⑴JUYOUJIAOXIAODEFENZILIANG。JINGYANBIAOMING,WEILEBIMIANZAIDNADECHUNHUAGUOCHENGZHONGFASHENGLIANDEDUANLIE,KELONGZAITIDEFENZIDAXIAOHAOBUYAOCHAOGUO10Kb。pBR322ZHILIZHEIZHONGXIAOFENZILIANGDETEDIAN,BUJINYIYUZISHENDNADECHUNHUA,ERQIEKERONGNAJIAODADEWAIYUANDNAPIANDUAN;

  ⑵JUYOULIANGZHONGKANGJUNSUKANGXINGJIYINKEGONGZUOZHUANHUAZIDEXUANZEJIHAO,NENGZHISHIZAITIHUOZHONGZUDNAFENZISHIFOUJINRUSUZHUXIBAOYIJIWAIYUANDNAFENZISHIFOUCHARUZAITIFENZIXINGCHENGLEZHONGZUZI。

  BIAOJIJIYINWANGWANGKEYIFUYUSUZHUXIBAOYIZHONGXINDEBIAOXING,ZHEIZHONGZHUANHUAXIBAOKEMINGXIANDIQUBIEYUFEIZHUANHUAXIBAO。DANGWOMENBAYIGEDNAPIANDUANCHARUDAOMOUYIGEBIAOJIJIYINNEISHI,GAIJIYINJIUSHIQULEXIANGYINGDEGONGNENG。

懂球帝  DANGBAZHEIZHONGZHONGZUDNAFENZIZHUANDAOSUZHUXIBAOHOU,GAIJIYINYUANLAIFUYUDEBIAOXINGYEJIUXIAOSHILE。YAOSHIRENGBAOLIULEYUANLAIBIAOXINGDEZHUANHUAXIBAO,XIBAONEIHANYOUDEDNAFENZIYIDINGBUSHIZHONGZUZI。HENXIANRAN,JIYAOZHISHIWAIYUANDNASHIFOUJINRULESUZHUXIBAO,YOUYAOZHISHIZAITIDNAFENZIZHONGSHIFOUCHARULEWAIYUANDNAPIANDUAN,NEIMEZHEIZHONGZAITIBIXUZHISHAOJUYOULIANGGEBIAOJIJIYIN。

懂球帝  LINGWAI,pBR322ZHILIZAITIHAIJUJIAOGAODEKAOBEISHU,ERQIEJINGGUOLVMEISUKUOZENGZHIHOU,MEIGEXIBAOZHONGKEJILEI1000~3000GEKAOBEI,ZHEIJIUWEIZHONGZUTIDNADEZHIBEITIGONGLEJIDADEFANGBIAN。

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